Synthesize and Functionalize of a Novel Organic-Inorganic Hybrid Material Based on Heteropoly Acid for Gene Delivery into the HEK-293T Cells

Gene therapy is a treatment option predicated on the underlying principle that disease can beaddressed by introduction of exogenous genetic material into somatic cells of patients for thepurpose of modulating gene expression of desired proteins [1] .Thus, the delivery of geneticmaterial, DNA and RNA, can be utilized to combat various acquired or heritable diseases andunder circumstances where natural immunity is abert has been reported here (Fig 1). In thisregard, we report the synthesis of a novel hybrid materials based on heteropoly acid (HPA)for gene delivery application. At the first, choline chloride was reacted with 2-chloroacetylchloride, and then heteropoly acid was added to the mixture. The synthesized organicinorganichybrid material was fully characterized and its biological application was investigatedin gene delivery into the HEK-293T cell line.