Investigation of mitochondrial tRNA leucine (UUR) gene mutations in Iranian patients with idiopathic repeated pregnancy loss

Background: Mitochondrial transfer RNAs (tRNA) genes are essential components of protein biosynthesis in mitochondrion. These genes are hotspots for mutations. These mutations are associated with a wide spectrum of human diseases. Many genetic factors are known to involve in repeated pregnancy loss (RPL).Objective: The aim of this study was analysis of tRNAleu (UUR) possible mutations in women with RPL.Materials and Methods: The nucleotide variations of tRNAleu UUR were investigated in 100 women with idiopathic repeated pregnancy loss. The related mitochondrial area was amplified using polymerase chain reaction (PCR). The PCR products were demonstrated by 2% agarose gel electrophoresis. Then all PCR products were run on SSCP gels, and samples showed considerable shifts due to nucleotide variations were sent for DNA sequencing. Sequencing results in order to further information, studied under bioinformatics websites and software.Results: The sequence analysis revealed no mutations in tRNAleu.Conclusion: Further study of an expanded series of these tRNAs mutations in a larger population sample is recommended to describe their possible etiologic role in idiopathic RPL.

کلیدواژه ها:

Mitochondria ، Mutation ، Repeated pregnancy loss ، mtDNA ، tRNAleu (UUR) ، PCR-SSCP