Fertilization failure following ICSI

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 672

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شناسه ملی سند علمی:

RMED08_167

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Male infertility can be resolved considerably by the intracytoplasmic sperm injection (ICSI), but the total fertilization failure (TFF) following this method occurs for about 3% of cases. It is very valuable to discover the causes of this complication and find a way to solve this problem.Objective: To discover the causes of fertilization failure following ICSI and find a way to solve this problem.Materials and Methods: To access related articles, PubMed, Science Direct and Google Scholar were used.Results: Total fertilization failure following ICSI may occur due to the defects in the oocyte, spermatozoa and can be due to technical problems. Defects in the activation of oocytes seem to be the most important reason, other factors include poor oocyte morphology, defects in M II oocyte spindle and sperm aster formation and premature sperm chromatin condensation. Treatment with artificial oocyte activation (AOA) may improve ICSI outcomes. There are several methods for AOA, including chemical, Physical and Mechanical activation. Chemical activation include calcium ionophores, such as ionomycin, Calcimycin (A23187) and puromycin increase membrane permeability to extracellular Ca2+. Physical activation (electro stimulation) forms pores in the plasma membrane. Mechanical activation can be acquired by aspiration of the oocyte cytoplasm may increase the oocyte calcium load at the time of injection. Sperm-related factors (morphology, motility, DNA integrity, viability) play important role in ICSI outcome. Morphologically selected sperm injection (IMSI), physiological ICSI (PICSI) technique for sperm selection can be used for patients with a history of TFF to reduce this complication.Conclusion: Fertilization failure following ICSI is a real challenge. The factors cause TFF should be carefully investigated and well documented to minimize the risk of recurrent TFF, and it seems IMSI combined by AOA is suitable to avoid TFF.

نویسندگان

F Tanhaye Kalate Sabz

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

M Ashrafi

Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

FS Amjadi

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Z Zandieh

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran