The effect of non-essential amino acid supplementation on the parameters and DNA fragmentation index of cryopreserved sperm

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 262

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شناسه ملی سند علمی:

RMED08_115

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Cryopreservation of spermatozoa is important because of new clinical demands. But sperm cryopreservation persuades serious changes in sperm function and sperm DNA integrity.Materials and Methods: This experimental study was performed on 40 semen sample from normal and oligoasthenoteratozoospermia men. In all volunteers, after 3 days of sexual abstinence semen sample were collected by masturbation in sterile containers. After liquefication the sperm parameters were analysed according to World Health Organization (WHO). Each sample was divided into two groups: one of them is frozen with non-essential amino acid supplement, another without non-essential amino acid. After a while the samples were thawed. Then sperm parameters were analysed and DNA damage were assessed by TUNNEL assay based on protocol.Results: This research showed that adding non-essential amino acid to cryopreserved semen can reduce DNA fragmentation index compared with control group.Conclusion: Growing evidences suggested that some antioxidant like vitamins or amino acid can reduce the effect of ROS-induced and cold shock damage.

نویسندگان

D Hamidi

Faculty of Sciences, Islamic Azad University Central Tehran Branch, Tehran, Iran