Prevalence of Helicobacter pylori vacA Genotypes in paraffin-embedded biopsy samples prepared from patients with upper gastrointestinal (GI) carcinoma

Introduction & Aim: Discovery of Helicobacter pylori by Marshall and Warren in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases is an infectious disease, and all consensus conferences were agreed that the causative agent is a bacteria. Prevalence of virulence factors varies according to patient disease, ethnicity, age, and country. H. pylori VacA causes vacuolation, pore formation, disruption of endo-lysosomal activity, apoptosis in gastric cells and immunomodulation. There is considerable genetic diversity among vacA alleles from different strains that lead to cancer. The aim of this study is Prevalence of Helicobacter pylori vacA Genotypes in paraffin-embedded biopsy samples prepared from patients with upper gastrointestinal (GI) carcinoma.Methods: Samples obtained from patients and DNA was extracted by QIAamp DNA Kit (Qiagen Inc., Germany) according to the manufacturer‘s instructions. PCR analyses were carried out to determine the presence or absence of vacA s1, vacA s2, vacA m1 and vacA m2 genes in each H. pylori positive sample. Results: showed that from all of 80 patients with gastric adenocarcinoma, The percentage of vacA genes (vacA s1, vacA s2, vacA m1, and vacA m2) are respectively 7.7%, 4.11%, 0%, and 0%.

کلیدواژه ها:

Helicobacter pylori ، vacA ، upper gastrointestinal carcinoma