Electrospun polycaprolactone -based nanocomposite scaffolds with tailored properties for tissue engineering application

Introduction Tissue engineering scaffolds have been known for their significant role in promoting cell adhesion, proliferation and eventually formation of the new functional tissue. In order to support the bone tissue regeneration, scaffolds should specified propertiessuch as: porous structure, biodegradability, appropriate mechanical strength, cell penetration capability, and osteoconductivity. Objectives In this study, polycaprolacton as a biocompatible polymeric matrix was composited with different nanoparticles such as carbon nanotube, graphene oxide, and layered double hydroxide to enhance structural and functional properties of electrospun scaffolds. Methods Attachment, proliferation and differentiation of rat bone marrow-derived mesenchymal stem cells (rMSCs) seeded onto the scaffolds were analyzed. The morphology and mechanical properties of the scaffolds were characterized using SEM and tensile test. Results The results indicated that the addition of nanoparticles enhanced the tensile strength. The bioactivity and degradation rate were also increased. Furthermor, attachment, proliferation and differentiation of rMSCs were significantly enhanced. Alkaline phosphatase activity demonstrated enhanced differentiation of cells on nanocomposite scaffolds

کلیدواژه ها:

Tissue engineering ، Scaffold ، Elecrospun ، Polycaprolactone