Developing a novel natural scaffold based on decellularaized amniotic membrane

IntroductionNowadays there are a lot of efforts to discover a biocompatible, cheap and available material for using in the bone repair. To address this issue amniotic membrane was selected, due to its inert characteristic for immune system. There are some previous studies shown, that minerals have the osteoconductive effect and could enhance the bone repair.ObjectivesIn this study the effect of mineralization on the decellularized amniotic membrane was investigated.MethodsAmniotic membrane was decellularized by a chemo-physical method. Then it was placed in the proximity of 5% gels containing calcium and phosphate produced in a ratio of 10 to 6. Saos2 cell line viability and morphology on the sheet was investigated by MTT test and SEM, respectively.Results: The MTT assay showed that the survival and growth of saos2 cell line were even more in mineralized sheet compare to the control sample. SEM and XRD data revealed the successful formation of minerals on the amniotic membrane and were mostly brushite, and in the SEM images, the cells were completely bonded to the surface of the sheet.ConclusionThe most important goal in tissue engineering is the optimization of the scaffolds. In this regard, biological simulation and imitation can be traced. In this study, we were able to construct a similar structure of bone with the preservation of potential biological properties.

کلیدواژه ها:

Tissue engineering ، Natural scaffolds ، Amniotic membrane ، Mineralization ، Bone repair