RAPD-PCR analysis of Candida albicans isolates from patients with OPC

Candida species are part of the normal microbiota of most people, but can also be responsible for infections, acting mainly as opportunistic microorganisms. The typing of Candida isolates is important for hospital epidemiology studies. The aim of this study was to analyze the molecular profile of Candida albicans isolates from individuals with OPC and verify genetic relationships between them using the RAPD-PCR methodology. A total of 50 C. albicans isolates were analyzed. Every isolate was typed by RAPD-PCR using the random primers RDS12 and CALB. The genotypic analysis showed similarity coefficient ≥ 90%, genotypes containing from 3 to 17 isolates, which encompassed 30 (58.82%) isolates and 13 (25.49%) genotypic particular strains. The first cluster, which was the smallest one, composed of ramification a (ATCC strain) and a (C32 and C35 strains), the second cluster composed of ramification b and b and the third one (the largest cluster) composed of 17 of 50 isolates which distributed in c and c ramification. This study reveals that the genetic diversity of C. albicans isolates from individuals with OPC may be complex.

کلیدواژه ها:

RAPD-PCR ، Candida albicans ، Candidiasis ، Genetic diversity.