Therapeutic effect of mesenchymal stem cells on acute kidney injury induced by bilateral ischemic reperfusion in rats

Background: Despite the advances in treatment methods for patients with acute kidney injury (AKI), the mortality rate in this patient has not reduced yet. Damage cause by reperfusion is one of the main causes of AKI. Mesenchymal stem cells (MSCs) are a stem cell population that widely used in basic and clinical trial studies. Stem cells are pluripotent cells that can transform into all cell types and repair the damaged tissues. Stem cells have different types and in this study we used bone marrow mesenchymal stem cell (BMSCs).Methods: This study was performed on 30 Sprague dally male rats, weighing 180-200 gr. The rats randomly divided into three groups: sham, Bilateral Ischemic Reperfusion (BIR), and BIR which received intraperitoneal bone marrow at 24 hours after surgery.Results: Histologic staining and pathologic examination of the kidney showed tissue destruction. Tissue repair was observed in the stem cell recipient group. BUN/CR level increased in the BIR group and in the BIR+ stem cell group lower than the BIR group.Conclusion: Using BIR method causes renal damage and increases the kidney toxicity and as a result, decreases renal function and is a good way to develop the AKI animal model. Bone marrow derived stem cells help repair damaged kidney tissue.