Plasmid related resistance gene in staphylococcus lugdunensis isolates from burn patients

Introduction: staphylococcus lugdunensis is emerging pathogens causing nosocomial infections with increasing resistance to antimicrobials. The purpose of this research was to identify antibiotic resistance plasmid genes for S. lugdunensis isolated from burn patients of hospitals in Amir-al-momenin.Methods: Total of 28 isolates were obtained from burn patients & detected by standard biochemical examinations. Antibacterial Resistance pattern was determination by disk diffusion (CLSI) .After phenotypic identification; the samples were extracted for plasmid using alkaline lysis with SDS. By using specific primers, the antibiotic resistance genes such as blaZ, dfrA, ermB, ermC, ileS, mphBM and msrA genes were tested by PCR method.Result: Based on the results for β-Lactamase production, it was found that ceftazidime and penicillins were generally associated with the highest frequencies of β-lactamase group antibiotics. According to the results for detection of Methicillin Resistance (Oxacillin Resistance) isolates, 11 (39.3%) were detected to have mecA-mediated oxacillin resistance. But in general, the most isolates had high-level resistance to Macrolides (erythromycin and Azithromycin). The results for the detection of inducible Clindamycin resistance it has been showed that a total of 23(82.1) isolates were resistant.Regarding genotypic analyses it has been indicated the following results msrA (27(96.4%)) blaZ (28(10%)), dfrA (28(100%)), ermB (15(53.6%)), ermC (26(92.9%)), ileS (8(28.6%)), and mphBM (27(96.4|).Conclusion: The plasmid plays a central role in facilitating genetic exchange and promote the acquisition and spread of antibiotic resistance genes.