The effect of Wi-Fi on change of gene expression associated to repair DNA including (CDK1AGADD45a) in placenta of pregnant mouse

Background: The effects of exposure to Wi-Fi signals on placenta were not evaluated previously. In the present study, we examined the effects of prenatal exposure to Wi-Fi signals on expressions of CDKNA1, and GADD45a in placenta and pregnancy outcome.Materials and Methods: This study was designed as a prospective trial which was conducted to examine the effects of prenatal (in uterus) exposure to Wi-Fi signals on expressions of CDKNA1, and GADD45a in placenta and pregnancy outcome in an animal model. Fifteen plug-positive females were assigned to each of the 5 groups. Pregnant mice in cases group were exposed to Wi-Fi signalassociated electromagnetic fields (2.4 GHz centrefrequency band), starting 5 days after mating and ending 1 day before the expected delivery, and exposure protocol were 2h-60cm, 4h-30cm, 2h-30cm, 4h-60cm. Mice in group control were served as the control, they were placed in Plexiglas restrainers and sham expose to Wi-Fi radiation (without energizing the Wi-Fi router) for 4 hours per day in term of 7 day at distance of 30 cm from the router.Results: Real-time quantitative PCR experiment showed that the relative fold changes of CDKN1A were upregulated at groups II-V (exposure protocols of 2h- 30cm, 2h-60cm, 4h-30cm, 4h-60cm) (1.51±0.25, 2.54±0.66, 1.75±0.33, and 2.07±0.32, respectively). In addition, the relative fold changes in GADD45a expression in intervention groups were significantly higher than that observed in the control group (1.37±0.29, 2.83±1.05, 1.62±0.26, 2.01±0.21, respectively in groups II-V, P < 0.001).Conclusion: In summary, our results provide evidence that overexpression CDKN1A and GADD45a in placenta tissue is caused by exposure to Wi-Fi radiation. However, further experimental studies are needed to investigate other genes and other aspects of pregnancy to determine role of Wi-Fi radiation on fertility and pregnancy.

کلیدواژه ها:

Pregnancy ، Wi-Fi radiation ، Placenta tissue