Genetic Diversity and Population Structure of Iranian tulips revealed by EST-SSR and NBS-LRR Markers
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 433
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شناسه ملی سند علمی:
JR_IJHST-4-2_003
تاریخ نمایه سازی: 15 تیر 1398
چکیده مقاله:
The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme combinations. According to EST-SSR genotyping, the average of gene diversity (GD) and polymorphism information content (PIC) were 0.66 and 0.62, respectively. However, the values of GD and PIC were equal for each NBS primer–enzyme combination which ranged between 0.85-0.95 with a mean value of 0.91. The mean value of resolving power (EST-SSR = 1.93; NBS-LRR = 17.39) indicated that the NBS markers had higher discriminatory power compared to the EST-SSR markers. UPGMA clustering confirmed the results of PCA which was further confirmed by Bayesian model-based STRUCTURE analysis. Population structure analysis detected 3 and 4 gene pools for 27 wild tulip germplasms with EST-SSR genotyping and NBS-LRR profiling, respectively. The AMOVA results indicated that molecular variation among populations (ΦPT = inter-population variation) was 82% and 93% of the total variation for EST-SSR and NBS-LRR markers, respectively. The results of this study will help the conservation and phylogenetic studies of tulips.
کلیدواژه ها:
نویسندگان
Ali Pourkhaloee
Ph.D. Student, Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
Morteza Khosh-Khui
Department of Horticulture, Faculty of Agriculture, Shiraz University, Shiraz, IR IRAN
Paul Arens
Department of plant breeding-Wageningen University
Hassan Salehi
Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
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