Nanozeolite A as a novel potential treatment for peptic ulcers

To date, prodigious efforts are being made to explore the potential biomedical applications of natural and synthetic zeolites. In the present study, considering the promising effects of zeolites both in prevention and treatment of various complications of gastrointestinal tract, we utilized a colorimetric approach to evaluate the potential inhibitory effect of nanozeolites on the Helicobacter pillory urease function; an enzyme responsible for various gastrointestinal complications such as dyspepsia and peptic ulcers.Various concentrations of nanozeolites A and X were tested for their urease inhibitory activity, using the common Berthelot assay as a rapid and effective test for detection of ammonia released by urease. Urease was preincubated with different amounts of the 1mg/ml stock solution of nanozeolites A and X in phosphate buffer (PH= 7.6) in 37°C for 15 min. Berthelot reagents were added to the samples and the absorbance of samples in were measured using a on a DB spectrophotometer at λ = 625 nm. The percentage of inhibition was calculated based on the concentration of ammonia in the samples which is a function of the activity of urease enzyme.

کلیدواژه ها:

Nanozeolites ، Urease inhibition ، Berthelot assay ، Helicobacter pillory