Cytotoxic evalution of chenopodium botrys essential oil on the cancer cell category of the tumor cervix( Hela)

Carcinoma of the uterine cervix is the second most common female tumor worldwide, surpassed only by breast cancer. Its incidence is disproportionately high (> 80%) in the developing world and its treatment of disease is usually palliative, amied only at symptom control [1]. Chenopodium botrys is a fragrant herb. In this research ,the toxicity of chenopodium botrys on cervical cancer (HeLa) essential oil was investigated.The seeds of chenopodium botrys was collected from sabzevar city ,and then essential oil was extracted by hydrodistilation. The cytotoxic effect of essential oil on the HeLa cell line was evaluated by MTT assay . the cultivated cells were exposed to various dilutions of (5, 10, 30, 50, 75, 100, 130, 170, 200) for 24 hours.The results were analyzed by Graphpad prism 6 software. The results showed that the essential oil of the chenopodium botrys has cytotoxic effects on the HeLa cell line in 24 hours (Fig . 1). The essential oil showed the highest cytotoxicity at a concentration of 200 μg/ml. The IC50 of chenopodium botrys essential oil was and 79.44 μg/ml.