Electrospun Soluble Eggshell Membrane Protein/Galatin/Polycaprolactone for Tissue Engineering Applications

In recent years natural polymers have been widely used in biomedical applications. Application of natural and biocompatible polymers in wound dressing, medical sutures and tissue engineering are extensively growing. Eggshell membrane (ESM) has potential as a natural scaffold because of its highly porous structure and good biocompatibility. The objective of this study is to prepare and evaluate a new type of soluble eggshell membrane protein (SEP)/ Galatin/PCL nanofibers using electrospinning method for tissue Engineering application. SEP/ Galatin/PCL nanofibers were successfully prepared with various blending ratios. The morphology, chemical composition, surface wettability, and mechanical properties of the nanofibers were characterized using scanning electron microscopy (SEM), contact angle measurement, Fourier transform-infrared spectroscopy (FTIR), and a universal testing machine. Results showed that the SEP/Galatin/PCL electrospun membrane was composed of uniform, bead-free nanofibers, which formed an interconnected porous network structure. The biological study results showed that SEP/ Galatin/PCL nanofibers could enhance cell attachment, spreading, and proliferation. The study indicated the potential of SEP/ Galatin/PCL nanofibers for Tissue Engineering application and provided a basis for future optimization.