Simultaneous determination and pharmacokinetic study of antidiabetic drugs by HPLC-UV using novel MSPE method

Herein, silica-coated iron oxide magnetic nanoparticle modified with imidazolium-based polymeric ionic liquid (Fe3O4@SiO2@PIL) was used as a sustainable sorbent for reliable magnetic solid-phase extraction (MSPE) and simultaneous preconcentration and determination of trace three antidiabetic drugs involving empagliflozin, canagliflozin and metformin in human plasma by HPLC-UV. The predominant factors affecting the extraction efficiency of the analytes were investigated in detail and optimized with one at a time approach. The limit of detections (LODs, S/N = 3) for empagliflozin, metformin and canagliflozin were 1.3, 6.0 and 0.8 ng mL-1, respectively. Reasonable linearity (0.997 ≥ r2 ≥ 0.993) and broad linear concentration ranges of 5.0–1200.0, 20.0-1800.0 and 5.0–1000.0 ng mL-1 were obtained for empagliflozin, metformin and canagliflozin, respectively [1]. Satisfactory intra-assay (3.8-7.5%, n = 9) and inter-assay (3.2-8.5%, n = 12) precisions as well as remarkable accuracies (≤ 9.1%) displayed good efficiency of the method [2]. Ultimately, the method was applied for the quantitation of low dose administration of invokamet in 4 healthy fed participants after oral administration and main pharmacokinetic data such as; T max: 3.2 ± 0.3 and 4.1 ± 0.5, C max: 390.4 ± 50.2 and 859.7 ± 91.2 for CANA and MET were achieved respectively.