Immobilized Lipase on graphene oxide as Biocatalyst for the Synthesis of Heterocyclic Compounds

Enzyme as an important group of biocatalyst is superior over chemical catalyst because of its high effectiveness, high specificity and green reaction condition. Among the enzymes from abundant sources, lipase have been widely used including enantioselective hydrolysis, chiral resolution, esterification, synthesis of enantioenriched monomers, and other enzymatic reactions[1]. For this propose, synthesis and chemical functionalization of GO@lipase: In order to prepare GO nanosheet, Functionalization of graphen oxide with APTES. lipase (lipase from Asperjillus Niger) was immobilized on to the GO@NH2 via covalent binding through GA activation procedure[2]. The synthesized nanobiocatalyst analysed by transmission electron microscopy (TEM), scanning electron microscope (SEM), IR spectroscopy, X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The scanning electron microscope micrographs for the GO nanosheet with bound lipase are shown in Figure 1. According to the SEM pattern, the surface morphology of coated sample ( GO@lipas ) in shown in Figure 1. This synthesized catalyst could be used in the synthesis of heterocyclic compounds.