Effect of solvent, time and method on the amounts phenol, flavonoid and the anitioxidant activity of Ixiolirion tataricum (Pall.) Herb extract

The application of natural and low-risk antioxidants is an unavoidable necessity.Natural antioxidants increase the strength of antioxidants of plasma. Phenol and total flavonoids are derived from secondary metabolites of plants have the potential to eliminate free radicals [1]. Solvent type, physicochemical properties of the solvents, extraction time and method are important parameters on extraction efficiency [2]. In this work, was studied effect of time (60, 90 and 120 min), solvent (H2O, CH3OH andn-hexane) and method (soxhlet and ultrasound- assisted) on total phenol content (TPC), total flavonoid content (TFC) and antioxidant activity (according to IC50, FRAPand BCB) for extract of Ixiolirion tataricum (Pall.)Herb. The plant (voucher number NP23/2-1) was collected in April of 2017 from Baba Amman Mountains of North khorasan Province in Iran. TPC and TFC were determined using the Folin-Ciocalteu and aluminum chloride methods; respectively. The mounts of TPC, TFC, IC50, FRAP and BCB in the optimum conditions are showed at Table 1. The results show that there is a significant relationship between TPC and the antioxidant activity. The highest amount for TPC, TFC, IC50, FRAP and BCB was found in H2O and CH3OH and the lowest value was observed in n- hexane. Also, the amount of TFC in CH3OH was more than other solvents, which can be related to the better extraction of flavonoid aglycones in CH3OH in addition to flavonoid glycones in this solvent