Using the CPE-scanometry method for the simultaneous preconcentration and determination of the Rhodamine B and Nile blue

In this work CPE-Scanometry as a fast, facile, cheap and sensitive method was used for simultaneous separation, preconcentration and determination of Rhodamine B and Nile blue in aqueous samples. Nile blue (or Nile blue A) is a fluorescent dye and a stain that used in biology and histology. It may be used with live or fixed cells, and imparts a blue color to cell nuclei [1]. Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acidfastorganisms, notably Mycobacterium. Since the rhodamine B cause to cancer and birth defect due to skin contact, so determination and preconcentration of these indicator dyes are important [2]. In this research we was measured the amount of the Rhodamine B and Nile blue simultaneously. Recently scanometry method has been noticed for the measurement of various analytes [3]. To improve the detection limit, this method coupled with preconcentration methods. In the study, was used triton X-114 as surfactant. Furthermore, experimental variables, such as, volumes of extraction and disperser solvents, pH, centrifuge time and round per minutes and salt addition were assessed and optimized with the aid of one variable at a time optimization methods.The method is based on the CPE of analytes from aqueous solution, diluting the extracted surfactant-rich phase with ethanol, transfer to Plexiglas® cell and scanning of the cells containing the analyte solution with a scanner and measuring the RGB parameters with software written in visual basic (VB 6) media.