Urea-based porous organic polymer/graphene oxide hybrid as a highly efficient sorbent for bovine serum albumin

In this work, a porous organic polymer (POP) was prepared via the reaction of tetrakis(4-aminophenyl)methane and 1,4-Phenylene diisocyanate [1]. The polymer was then combined with graphene oxide (GO) [2], to prepare POP/GO as a novel and highly efficient sorbent for pre-concentration and extraction of serum albumin samples, the most abundant protein in plasma [3], prior to spectrophotometric determination. Parameters which influence the extraction efficiency such as the amount of the adsorbent, pH of sample solution, ionic strength, adsorption and desorption time were investigated and optimized. Detection limit of the method 0.068 mg L-1 and determination coefficients (R2) of 0.9991 were obtained. The intra- and inter-day was calculated with five replicates in the same day and seven consecutive days, respectively. Intra-day and inter-day precisions were 1.7% and 5.9%, respectively. The maximum sorption capacity was 357.1 mg g-1. The proposed method was demonstrated to be sensitive enough for determination of serum albumin from biosamples.