Magnetic Fe3O4/SiO2 Nanoparticles for Ciprofloxacin Detection in Biological Samples

Ciprofloxacin, chemically 1,4-dihydro-1-cyclopropyl-6-fluoro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid (Fig. 1), is a quinolone antibiotic drug with a broad spectrum of activity against a variety of gram positive and gram-negative bacteria. Several methods have been reported for the quantitative analysis of the ciprofloxacin, such as titrimetry1, HPLC2 and AAS3 methods. The present work aims to develop a highly sensitive, simple and rapid UV-Vis spectrometric method for application in quality control analysis. This study has been offered a solid phase extraction method based on functionalized magnetic nanoparticles. This method is simple, fast and affordable to extract ciprofloxacin from aqueous solution. Synthesis of functionalized magnetic nanoparticles of iron and sodium hexafluorosilicate is done with the help of co-precipitation method. Detection was performed at 275 nm, the maximum wavelength of ciprofloxacin. In this condition, linear range was achieved in two areas. For low concentrations of 0.1 to 1 and 1-20 (mg/L) for high concentrations were evaluated. The method limits of detection and limits of qualification were 0.0472 and 0.157 (mg/L) for low concentrations and 0.458, 1.52 (mg/L) for high concentrations, respectively. Also in optimal conditions, the concentration of ciprofloxacin in real samples of blood were determined and relative recoveries for real samples were evaluated too.