The investigation of chitosan effect on phenolic compounds and antioxidant activity of in vitro cultured Salvia leriifolia

Salvia leriifolia Benth (Lamiaceae family) is native of tropical areas of South Khorasan, Semnan and part of Afghanistan with various medical properties including antimicrobial and anti-fungi effects, anti-diabetics, anti-pain and anti-inflammatory characteristics that are dependent on secondary metabolites including terpenoids, saponins, flavonoids, tannins, and alkaloids. The use of elicitors such as chitosan in the tissue culture is one of the main methods for increasing the production of secondary metabolites. The aim of the present study was to investigate the effect of various concentrations of Chitosan (10, 50 and 100 mM) on secondary metabolites and antioxidant activity of S. leriifolia in in vitro condition. Phenolic and flavonoid contents have been measured by Folin-Ciocalteu and chloride aluminum methods respectively. Antioxidant activity was investigated by DPPH method. The results indicated that the total phenol and phenaloid rate and the antioxidant activity significantly increased compared to the control group and the highest rate was observed by 10 mM chitosan. Thus, by optimization of chitosan concentration, it is possible to increase antioxidant properties of S. leriifolia.

کلیدواژه ها:

Chitosan ، Secondary metabolites ، Antioxidant activity ، Salvia leriifolia