Isolation and identification of an Actinomycete isolate capable of producing anti-MRSA compound from soil samples and optimization of production in liquid culture

The increasing resistance to commonly used antibiotics has become a major public health concern. This phenomenon can lead to higher treatment costs and length of stay in the hospital. Purpose of this study Streptomycess trains isolated from soil samples of native Morphological and biochemical basis of their inhibition effect against MRSA, high chosen the isolation, Therefore, further molecular identification and optimization of the production of antibiotic substances. Ten strains of actinomycetes based on morphological characteristics and uses a semidifferential medium were isolated and purified. Three field isolates identified by morphological characteristics, biochemical and molecular (16S rDNA) was performed. Drug Susceptibility Testing of MRSA cases were antibiotic disk diffusion method than 6. Antagonistic effect of Dvmtdpen isolated-Linear and agar well diffution were used. Four solvent extraction method of antibiotic liquidliquid used and the dried extract wasre-dissolved, and agar well diffution method was investigated. Three antagonistic actinomycetes against MRSA isolates showed that the growth inhibitory effect of two strains of considerable value. The most effective antibiotic isolated from cultures obtained on the seventh day of the etherextract showed the best effect. Ten isolates identified by conventional methods and by molecular methods and PCR amplification of 16S rDNA sequencing and comparison with gene bank was completed. The soil can bean important source of actinomycetes produce antibiotics against organisms that are resistant. Researchers believe that such a plan because the country needs to obtain new sources of bacteria to antibioticsis necessary.

کلیدواژه ها:

PCR ، 16S rDNA ، Streptomyces ، Methicillinresistant Staphylococcus