Study of the expression of isopiperitenone reductase gene in peppermint (Mentha piperita)

Peppermint (Mentha piperita) has been considered due to the presence of menthol secondary metabolite. The isopiperitenone reductase enzyme plays an important role in the production of menthol, and decreasing or increasing the expression of the isopiperitenone reductase gene leads to a decrease or increase in the amount of menthol. The amount of menthol production in this plant is heavily influenced by environmental factors. In order to investigate the effect of salinity, drought and temperaturestress on the amount of isopiperitenone reductase gene expression in the pathway of menthol biosynthesis, 36 peppermint plants under drought (0, 50, 100 and 150 mM mannitol), salinity (0 , 50 and 100 mM sodium chloride) and temperature (23, 26 and 29°C) were cultured. After two weeks, RNA extraction and cDNA synthesis were performed and the gene expression level was evaluated using Real-Time PCR. The results showed that with increasing mannitol and sodium chloride level, a significant decrease was observed in gene expression, but with increasing the temperature from 23° to 29°C, there was no significant decrease in the expression of isopiperitenone reductase gene. Also, the results showed increasing the temperature from 26°C to 29°C, there was a significant decrease in the expression of isopiperitenone reductase gene. Overall, the results of this study show that the expression of this gene decreases with increasing temperature, drought, and salinity

کلیدواژه ها:

Drought ، Isopiperitenone reductase ، Peppermint ، Salinity ، Temperature