Isolation and identification of the c-type lysozyme-encoding gene from Salmo trutta caspius

The innate immune system of fish is considered to be the first line of defense against a broad spectrum of pathogens. Lysozymes are key proteins of the innate immune system against bacterial infection. Non-specific antimicrobial agents, such as lysozyme, may be more important in fish comparing tomammalsbecause fish apparently has a less developed specific immune system. Salmonid fishes have long been of great interest due to the commercial importance as model systems for addressing a wide range of evolutionary and ecological question. In this study, we report the isolation, amplification, and characterization of chicken-type (c-type) lysozyme gene from Salmo trutta caspius. After total RNA extraction from the apical compartment of the fish kidney using TRIzol reagent, complementary DNA (cDNA) was synthesized by cDNA Synthesis kit. The cDNA was used as a template for the PCR using specific primers that annealed to the conserved regions of the lysozyme gene from another genus of Salmonidae family. Analysis of the sequence of amplified gene revealed that the cDNA contains an open reading frame (ORF) of 432bp, encoding 143 amino acid, with 97% identity toLysozyme C of Rainbow trout(Oncorhynchusmykiss).

کلیدواژه ها:

Salmo trutta caspius ، Immune system ، Lysozyme C ، cDNA synthesis ، Gene amplification