The study of resistin gene expression changes in adipose and stomach tissues of male rats subjected to chronic immobilization stress

Chronic immobilization stress disrupts the function and physiology of the body. Resistin is a hormone that produced from adipose tissue. Increasing the expression of resistin gene is associated with metabolic syndrome. The aim of this study was to determine the changes in the expression of resistin gene in the adipose and stomach of tissue rats exposed to chronic immobilization stress. 10 male Wistar rats were divided into groups of Control and chronic immobilization stress. Rats group of chronic immobilization stress were exposed to 6 hours for 21 consecutive days. At the end of this period, animal adipose and stomach tissues were removed. Further, RNA extraction and cDNA synthesis were performed and changes in the expression level of resistin gene in these tissues were evaluated quantitatively by Real-Time PCR.The results of the data analysis show that chronic immobilization stress increased the expression of resistin gene in adipose tissue and decreased its expression in the stomach tissue compared with the control group. Chronic immobility stress causes a metabolic syndrome with resistin gene disruption. Chronic immobility stress has increased resistin in adipose tissue. Increasing resistin causes changes in glucose levels and increased insulin resistance. The most important effects of resistin increase are inflammation. Chronic immobilization increases the inflammation of the rats by increasing the expression of resistin.

کلیدواژه ها:

Resistin ، Chronic immobilization stress ، Rat