Purposeful transfer of polyethyleneimine polymer using magnetic nanoparticles and static magnetic fields to normal and cancerous cells

Magnetic nanoparticles have the ability to function at cellular and molecular levels due to their properties, including magnetic properties, which has made them an attractive vector for delivery of medication. Cationic polymers, such as polyethyleneimine, are positivepolymeric polymers that are active in active groups and have therefore been used extensively in biology, and their most important application is gene transfer. The main problem with these polymers is their biodegradability, which induces cell toxicity. Studies show that polyethyleneimine polymer, by binding to negative pregnant proteins within the cell, leads to induction of apoptosis. The purpose of this study was to transfer polyethylene immune polymer as a drug using magnetic nanoparticles for induction of cell death of cancer cells. To this end, the binary polyethylene amine-magnetic nanoparticle complex (MNP-PEI) was formed by attaching magnetic nanoparticles of iron oxide synthesized to the cationic polymer and confirmed by FTIR test. In the following, normal fibroblast cells and breast cancer with different concentrations of binary complex and polymer were treated only in presence and absence of a staticmagnetic field. The rate of cell death in different treatment groups was calculated using the MTT test. IC50 of different groups was calculated. Results showed that in the presence of a magnetic field of binary complex (MNPPEI), compared to polymer only, the percentage of cell survival significantly decreased.

کلیدواژه ها:

Magnetic nanoparticle complex ، Polyethyleneimine ، Gene transfer