Investigation of the inhibitory effect of two new phenol-ninhydrin derivatives on humansalivary α-amylase enzyme

Inhibition of α-amylase, an enzyme that plays a role in the digestion of starch and glycogen, is considered asa strategy for the treatment of disorders in carbohydrate uptakes,such as diabetes and obesity. Inhibitors of carbohydratedigesting enzymes, such as alpha-amylase and alphaglucosidase, are now actively searched for since they could ultimately make useful medicines against diabetes and obesity. In the present study, by considering the structural requirements, two new phenol- ninhydrin derivatives as α-amylase inhibitor were synthesized. Inhere, pyrogallol has been used as a polyphenol compound. Ninhydrin-Pyrogallolmonoadduct andNinhydrin-Pyrogallolbisadductderivativescharacterized by spectral analysis and finally evaluated for the inhibition of human salivary α-amylase activity by the method of Bernfeld. Mono and bis adduct derivatives exhibited their best α-amylase inhibitory activity at a 1 millimolar concentration (23and 69.4 Percentageof inhibition). Results showed that bis adduct derivative can be explored as a strong anti-hyperglycemic agent. Putative binding mode of two derivatives with the target enzyme was also explored by the docking studies

کلیدواژه ها:

Diabetes ، Human salivary alpha-amylase ، Ninhydrin pyrogallol derivatives