Tyrosinase inhibitory and antioxidant activity of methanolic extract from various aerial organs of Astragalus siliquosusBioss and Verbascum phoeniceum L.

The process of melanogenesis is responsible for the pigmentation of the skin, eyes, and hair of human.Melanin production is also responsible for the browning of fruits, vegetables, and fungi. Melanogenesis begins with the oxidation of L-tyrosine to L-Dopa by tyrosinase .The accumulation of melanin in the skin causes complications such as skin lesions, eczema, and melasma in humans . Inhibition of tyrosinase could be effective in treatment of those complications.The aim of this study was to determine inhibitory activity on tyrosinase and antioxidant activity of methanolic extract of various aerial parts of Astragalus siliquosusbioss and Verbascum phoeniceum L.Methanolic extracts of the isolated organs of the plants were prepared using rotary evaporator. Inhibitory effects of the extracts were evaluated in nine concentrations in 96 wells at 492 nm wavelengths using a microplate reader. The antioxidant activity of the extracts was evaluated using DPPH free radical scavenging index.The most inhibitory activity of the Astragalus siliquosusBioss was at 1000 mg/ml concentration of its flower extract (97% inhibition and IC50 = 1.58 mg/ml) and 1500 mg/ml concentration of its stem extract (100 mg / ml and IC50 = 13.225 mg/ml). For Verbuscumphoeniceum L. the highest inhibitory activity was observed in the 1500 mg/ml concentration of its leaf extract (87% inhibition and IC50 = 155.3 mg/ml). All aerial parts of the Astragalus siliquosus had 100% antioxidant activity with EC50s of 0/089, 1/775, 1.25 mg/ml for its flower, leaf, and stem respectively. On the other hand, just leaves of the Verbascumphoeniceumhad a 100% antioxidant activity, with EC50 of 0.129 mg/ml. According to the results of this study, methanolic extracts of flower and leaf organs of Astragalus siliquosusBioss, and just leaf organ of Verbascumphoeniceum L. had a reasonable inhibitory effect on tyrosinase activity. So feature studies could be focused on those organs to separate potential agents with pharmaceutical and food industrial applications

کلیدواژه ها:

Tyrosinase ، Inhibitor ، Methanolic Extract ، Astragalus siliquosus ، Verbascumphoeniceum