Determination of the effect of cinnamaldehyde of cinnamon on the urease activity

Urease-producing bacteria have a negative effect on human health and the environment. Forexample, Helicobacter pylori is a pathogenic bacterium that is associated with infection and peptic ulcer. Pathogenicity is mediated by urease. Cinnamaldehyde is the most important compound of cinnamon essential oil, which has anticancer and antimicrobial properties. Cinnamaldehyde effect on the urease activity is examined in this study. The activity of urease in the presence and absence of cinnamaldehydewas measured spectrophotometrically by microplate reader during two minutes which was required for releasing enough ammonia to raise the pH of the assay mixture. Phenol red was used to indicate ammonia production with a color change from yellow to pink that is measured at the wavelength of 570 nm. Cinnamaldehyde inhibited the activity of urease after 5 minutes of preincubation which caused an increase in the Km value while the Vmax value remained constant. Inhibition decreased as a result of the substrate concentration rise. Consequently, in this study cinnamaldehyde is described as a competitive inhibitor of urease. Inhibition of urease enzyme by cinnamaldehyde may play an important role in the antimicrobial activity of cinnamon extract as well as in cinnamon s medicinal properties against peptic ulcer.

کلیدواژه ها:

Urease ، Cinnamaldehyde ، Enzyme assay ، Enzyme competitive inhibition