Investigation of absorption wavelength changes in Griess microassay for detection of nitric oxide

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 499

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شناسه ملی سند علمی:

BIOCONF20_373

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Griess colorimetric assay is based on azo dyes, which has changed a lot today. This simple and inexpensive method is used indirectly to detect nitric oxide (NO) in biological and non-biological samples by means of RNI (Reactive Nitrogen Intermediates). In this study, we evaluate some wavelengths to improve this colorimetric assay using the Griess microassay (GMA). Nowadays, the increasing use of this assay in laboratories and research centers and even kits have encouraged the researchers to study the physical factor of optimized wavelength based on the Griess assay. Although different wavelengths have been reported based on this essay, it is still ambiguous to find out which wavelength brings us closer to the best results in measuring RNI. First, the Griess solution was prepared, and then the sodium nitrite solution, prepared at specified concentrations was added to draw the standard graph of linear regression. Finally, wavelengths 480, 490, 500, 510, 520, 530, 540 were read and evaluated using a microplatereader. Linear regression analysis at 480-510 wavelengths had an increasing trend so that we observed a reduction in linear regression as the wavelength changed from 510 to 540 nanometer (nm). By studying different wavelengths in the GMA for detecting of NO to optimize it, the best linear regression observed at 510 nm wavelength, which showed the highest absorption in the standard curve, leading for best detection of NO by GMA

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نویسندگان

Soheila Shir Mohammadi

Department of Biochemistry, Islamic Azad University of Shiraz, Shiraz, Iran

Hossein Nahrevanian

Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran

Nematollah Razmi

Department of Biochemistry, Islamic Azad University of Shiraz, Shiraz, Iran