The evaluation of antibiotic resistance pattern and frequency of extended-spectrum betalactamases among clinical isolates of Pseudomonas aeruginosa isolated from inpatients of Imam Khomeini hospital,Kermanshah, Iran

Pseudomonas aeruginosa is one of the most important cases of nosocomial infections. The resistance of this bacterium to different antibiotics especially to beta-lactams and carbapenems is increasing. The aim of this study was to determine of antibiotic resistance pattern and extended spectrum beta-lactamases frequency in Pseudomonas aeruginosa isolates. In this cross-sectional study, a total of 66 Pseudomonas aeruginosa isolates from Imam Khomeini hospital in Kermanshah were collected. The sensitivity of isolates using agar disk diffusion method (Kirby- Bauer) to followed antibiotics: ceftazidime (30μg), cefotaxime (30μg), imipenem (10μg), kanamycin (30μg), co-trimoxazole (25μg), ciprofloxacin (5μg), ceftriaxone (10μg), clindamycin (30μg), Cefoxitin (30μg), gentamycin (10μg), was determined. The ESBL producing isolates were determined using the combined disk and frequency of PER and OXA beta-lactamases genes by using PCR was evaluated. Among 86 isolates of Pseudomonas aeruginosa,the highest rate of resistance was related to cefotaxime, cotrimoxazole, kanamycin, ceftriaxone, clindamycin, Cefoxitin, ceftriaxone was 100% and resistance to gentamicin, imipenem, ciprofloxacin, and ceftazidime was 87.27 and 83.33 and 81.80 and 80.30% respectively. The frequency of phenotypic ESBL producing of isolates was 91.91% and frequency of OXA was 87.5% and frequency of PER gene was 78.12%. The results of this study showed the high rate of resistance to different antibiotics among Pseudomonas aeruginosa isolates so it’s necessary to improved treatment methods

کلیدواژه ها:

Extended-spectrum beta-lactamases ، Pseudomonas aeruginosa ، Antibiotic resistance ، Kermanshah