Effects of Silver Nanoparticles on Tissue Culture of Aloe vera (Aloe barbadensis)

Aloe (Aloe barbadensis) is one of the most valuable economic and medicinal plants. The natural reproduction of this native Iranian plant is slow, it can not meet the needs of the country s pharmaceutical industry. In this study, the effects of silver nanoparticles on plantlet propagation in vitro of Aloe vera (Aloe barbadensis) were investigated. Aloe vera meristem was cultured on MS medium (Murashig, Skoog, 1962) containing Benzoyl amino-purine (3 mg/l) and Indol Acetic Acid (2 mg/l) hormones, and morphological traits (stem length, Number of stems, root length and number of roots). In vitro plantlets were investigated in treatment of silver nanoparticles with five levels including zero (0, (control), 5, 10, 15, 20 mg/l) during 2 subcultures. Statistical analysis of the data was performed using one-way ANOVA at the probability level of 0.05 with SPSS software (Ver.22). The average stem length (8 cm) and proliferation rate (5 plantlets) regenerated from the Aloe vera meristem. The regenerated plantlets in the treatment of silver nanoparticles had the highest mean shoot length in the treatment of 5 mg/l with a probability level of 0.05. Reduction of number of stems in treatments of silver nanoparticles decreased compared to control (0 mg/l) and the highest. The length and number of roots in the treatment of 15 mg/l were more than 0.05 in the control treatment. Different concentrations of silver nano particles caused the morphological changes of Aloe vera plantlets in vitro.

کلیدواژه ها:

Nanoparticles ، Aloe vera plantlets