Optimization of beta-carotene production by yeast strains from a waste leather factory

Yeasts have a special value for human in biotechnology because of the production of pigments. Rhodotorula species produces high amounts of beta-carotene. The aim of this study was to maximize the production of betacarotene at least prices from native yeast species. The four isolated studied were isolated on specific environments during three stages of sampling. Subsequently, using biochemical test and The PCR method two isolates Aa1 and Aa4 were identified. The production of beta-carotene was determined by isolating identified by standard strain in different conditions of salt, nitrogen source, carbon source, aeration, temperature, pH. Optical pigment absorption at470 nm was read by the spectrophotometer. Among the four isolates, only the isolate Aa1 produces a carton-free pigment. The genetic identification of the two isolates Aa1 and Aa4 was similar to those of Rhodotorula mucilaginosa and Debaryomyces hanseni, respectively. The results showed that, the maximum production of carotene was obtained after optimization of 75.6 μg / ml for Rhodotorula mucilaginosa and 32.7 μg / ml for Rhodotorula glutinis standard strain. The isolation of native species and the optimization of their functional activities in the laboratory is not only useful in the production of high-quality industrial products, but also the use of native species is very economical

کلیدواژه ها:

Carotinoide ، Beta-carotene ، Antioxidant ، Rhodotorula