Evaluation of Thiazole-derivatives cytotoxicity effects on breast cancer line (MCF-7) and human non-small-cell lung cancer (A549)

Cancer is a group of diseases characterized by the uncontrolled cell growth and division, the second leading cause of death worldwide and among more than 100 types of cancer, breast cancer is leading cancer diagnosed in women and lung cancer is one of the most common cancers in men. Due to the progression of cancer, drug resistance, and side effects of current medications, researchers are looking for new synthetic drugs with strong effects on cancer treatments and fewer side effects on cancer patients. In this study, a series of synthetic compound Thiazole derivatives with anticancer activity (8 samples that named C1-C8) were tested. In this regards, the cell viability effect of 8 samples of Thiazole derivatives in different concentrations of 100μm to 1000μm with a three-time repeat count on breast cancer line MCF-7 and Human non-small-cell lung cancer A549 cancer cell line have been investigated. Etoposide as a positive control and DMSO as negative control have been used to determine the accuracy of the work. The effect of compounds treatment on the viability of MCF-7 and A549 cells were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). MTT assay was used for the assay to measure the toxicity of the drugs. Our results showed that in MCF-7 cells, among the 8 available samples, IC50 was found in sample C7 at a concentration of 300μm and in A549 cells, IC50 was found in samples C1 at a concentration of 400μm. our results could provide more insights and shed further light on the in cancer therapy approach.

کلیدواژه ها:

Cytotoxicity ، MTT assay ، Synthetic anticancer drugs