Study of biodegradation of oil pollution in laboratory grade

The effects of oil pollution on human health have led to the provision of solutions. Identification of oil-degrading bacteria can convert heavy hydrocarbons into simpler derivatives. Soil samples collected from 5 polluted areas. One gram soil was inoculated with 100 ml of synthetic medium containing at least 1% oil as the source of carbon and incubated at 200 rpm for 5 days. After observing the turbidity in the broth medium, 100 μl was transferred to agar medium containing 1% oil. For purification single colonies were cultured in an agar medium containing 1% oil. The morphological characteristics of the strains were described by Gram staining and, for the analysis of the rate of degradation, the residual oil was separated by diethyl ether. The weight of oil was decreased in comparison to the initial weight of oil. Eleven strains of gram-negativeand 1 strain of fungi were identified. Out of 12 isolated strains, 2 strains showed the highest degradability, which included strains screened from Shahrey refinery (64%), Jayrood repairer (57%), wastewater from the Moon-Urban refinery (52%), Samen Al-Hajj Square Bus Terminal (47.57%), ethanol purifier flood in Shahr Refinery (44.7%). These isolates are Gram-negative bacilli. The strains can play a role in potential the reducing of pollution at the field scale. This method of studying is easier, less costly and faster than spectroscopy and chromatography.

کلیدواژه ها:

Biodegradation ، Liquid petroleum ، Oildegrading bacteria ، Oil pollution