The study of OeACP2 polymorphism in some Iranian native olive cultivars

Olive (Olea europaea) genus from Oleaceae family has about 20 shrub species which have been spread around the world. Olive oil is valuable not only for its natural resource but also for unsaturated fatty acids content, particularly oleic acid, which made it more stable than other vegetative oils. Desaturase enzymes such as Stearoyl-ACP desaturase, desaturates the saturated fatty acids. Acyl-carrier protein is one of the most protected proteins and is considered as hydrocarbon chains transporter in vivo. Most recently six ACP coding cDNAs were characterized from Olea europaea, which two of them, OeACP2 and OeACP3, showed the most occurrence in the fruit, leaf and flowers, respectively. Conversely, results demonstrated that OeACP2 and OeACP3, less than the former, alleles were involved in fatty acids biosynthesis and tri-acyl glycerol accumulation in fruits. In this research the quality and quantity of oil of Iranian olive germplasm evaluated by Firestone (1989) method and after recognition of the eligible genotypes (Mary and T18 as qualified and Shengeh and T20 as less qualified) OeACP2 gene was isolated from them and sequenced. Then the nucleotides sequence and possible efficient polymorphisms in coding regions was studied by bioinformatic softwares. The proposed proteins was studied according to their structural stability and function, bioinformatically. We found 19 nucleotide polymorphisms in exons 2 and 3 of OeACP2 that 11 cases were related to the high quality oil varieties and no significant structural changes were observed in the protein analysis.