The Nucleotide Analysis of Reverse Transcriptase (RT) Enzyme Gene in Escherichia coli isolated of Urinary Tract Infections

Background. Parts of the Escherichia coli’s genome caused urinary tract infections, include MsDNAs, the retrons, and the Reverse Transcriptase enzyme gene,and are referred as Orphan genes, Lacking origin, and performance in different strains of this bacterium. Retrons play a role in production of RT (Reverse transcriptase) enzymes.the RT was generally an eukaryotic enzyme,but it has also been observed in bacteria. Methods. In this study, 200 samples of urinary tract infections were collected from Tehran, Rasht, Tonekabon and Sari. After DNA extraction by using Boiling method and commercial kits (ROCHE), designing and synthesizing specific primers, the presence of RT gene was investigated by PCR, electrophoresis and sequencing. Results. The results of PCR and Melting Curve Analysis (HRM) indicated that primers designed in this study could identify the full specificity of the RT gene and confirm its presence in E. coli.out of 200 specimens, 27 samples had RT gene. We found that the sequence of this gene was not different in terms of nucleotide analysis in different strains, and was retained in all bacteria Conclusion. Identification, Nucleotide Analysis and sequencing of the orphan genes that are part of the non-homologous genes are necessary to compare with other sequences and to understand the origin and functions of these genes. Considerable results are selective pressure on the gene encoding the RT enzyme in E. coli.

کلیدواژه ها:

Escherichia coli ، MsDNAs ، Orphan genes ، Retrons ، RT (Reverse Transcriptase)