Menstrual Blood Stem Cells Show Higher Efficiency in Comparison with Their Conditioned Media in Repair of CardiacFunction in Rat Model of Myocardial Infarction

Background and Aim: Myocardial infarction (MI) assumes to be a majorpublic health problem in the world. The aim of the present study wasto compare the effects of administration of menstrual blood stem cellsconditioned media (CM) with menstrual blood stem cells (Mens SCs) oncardiac function in myocardial infarction in rats as an animal model.Methods: For this purpose, forty male Wistar rats were randomlydivided into 4 groups: 1) sham, 2) myocardial ischemia (MI), 3) MensSCs received, and 4) CM received. The animals were anesthetized with80 mg/kg of ketamine and 20 mg/kg of xylazine intraperitoneally andplaced under positive pressure ventilation. After thoracotomy, the heartwas exposed and the left anterior descending (LAD) coronary artery waspermanently ligated by 0-6 prolene suture. 30 minutes after ligation andinduction of MI, the animals belonged to the MI and CM groups receivedan intramyocardial injection of 120 μL phosphate-buffered saline (PBS)and CM into the peri-infarct zone at 3 points, respectively. The animalsof group 3 received 1.5×106 Mens SCs suspended in 120 μL Mens-CM at4 sites (30 μL per each site) in the borders of MI region. Sham-operatedrats were submitted to an identical procedure except for LAD ligation.Echocardiography and histopathological evaluation were performed atday 7 and 28.Results: The results demonstrated significant scar size reductionand increased ejection fraction (EF) and fractional shortening (FS)after Mens SCs therapy (P<0.05) however, the group received CM,showed no significant improvement in myocardial infarction (P> 0.05).Histopathologic studies in the group received Mens SCs showed adecrease in the amount of fibrosis and also relatively preservation ofthe cardiomyocytes in the ischemic region. In the CM –received group,in the infarct area, most cardiomyocytes were lost and lysed. Scar sizewas significantly smaller in Mens SCs received group compared to theCM-received group (P ˂ 0.05). Interestingly, cartilage metaplasia wasobserved in MI and the CM-received groups, considered as a negativeconsequence of tissue regeneration in severe cardiac ischemia. However,Mens SCs therapy sounds to protect from this change.Conclusion: Our findings indicated that application of Mens SCs couldimprove cardiac structure and function in animal models of myocardialinfarction

کلیدواژه ها:

Menstrual blood stem cells ، Conditioned media ، Rat ، Histopathology ، Echocardiography