Neuroprotective and Neurodifferentiative Properties of Ferulic Acid
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 356
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شناسه ملی سند علمی:
NSCMRMED03_240
تاریخ نمایه سازی: 30 دی 1397
چکیده مقاله:
Background and Aim: Ferulic acid (FA) is a phenolic compound withknown anti-apoptotic and anti-oxidative properties. Effect of FA on theinduction of neurogenesis and neuro-differentiation beside its effect onneuroprotection makes this compound very interesting for stem cellresearches. We designed this set of experiments to evaluate the effect ofFA on neuronal differentiation and neuroprotection.Methods: ROS-mediated apoptosis was induced on PC12 cells by usinghydrogen peroxide then with the treatment of different concentrationsof FA was started. To evaluate the differentiation-inducing the effect ofFA, PC12 cells and mouse neural stem cells (mNSCs) were treated withdifferent concentrations of FA. MTT, quantitative real-time RT-PCR andimmunostaining assays were performed on cells.Results: FA treatment at low concentrations significantly reduced theapoptosis rate in treated PC12 cells. Real-time RT-PCR and western blotassays confirmed that FA revealed this effect through stabilization anddegradation of P53 by increasing the expression rate of SIRT1, SIRT7and MDM2 and down-regulation of USP7. Beside this anti-apoptoticeffect, FA treatments on PC12 cells and mNSCs at higher concentrationson PC12 cells and mNSCs increased their differentiation toward matureneurons. Immunocytochemical staining against beta-tubulin III andMap2 verified the presence of mature neurons, and western blot assay onFA treated PC12 cells showed a stepwise rise of phosphorylated-ERK1/2 as the concentration of FA was increased.Conclusion: our findings showed that FA at low concentrations has aneuroprotective effect through up-regulation of SIRT1, SIRT7, andMDM2, and in higher concentrations can promote neural differentiationand neurite outgrowth.
کلیدواژه ها:
نویسندگان
Farshad Homayouni Moghadam
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Mehrnaz Mesbah Ardakani
Imam Hossein Hospital of Sepidan, Shiraz University of Medical Sciences, Isfahan, Iran
Mohammad Hossein Nasr-Esfahani
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran