Infected Carrier Cells: A New Strategy for Systemic Delivery of Oncolytic Reovirus in Cancer Cell

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 354

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شناسه ملی سند علمی:

NSCMRMED03_189

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Cervical cancer accounts for the most commoncancers in women, underlining the need for the development of newtherapies. Reovirus is a naturally oncolytic virus, selectively kills tumorcells. Studies have shown limited antitumor efficacy due to insufficientviral delivery. Mesenchymal stem cells (MSCs) possess the ability toprotect from circulating neutralizing antibody and specifically home intotumor sites. This property of MSCs could be exploited for the delivery ofvarious anti-tumor agents directly into tumors. We explored the effects ofreovirus loaded into MSCs on TC-1 tumor model cells.Methods: In this experimental study, MSCs derived from adipose tissuewere infected with reovirus T3D. After co-culturing of MSCs-loaded withreovirus in TC-1 cell line, we assessed the ability of these cells to deliverthe virus to target cell line and the effect on cell death was measured byreal-time PCR.Results: In this study, Adipose-derived MSCs (AD-MSCs) that wereinfected with oncolytic reovirus, delivered the virus in co-cultured TC-1cells. Reovirus-infected MSCs induced a higher level of apoptosis in TC-1cells compared with the apoptosis induced by their direct infection withsimilar virus titer.Conclusion: AD-MSCs-loaded with oncolytic reovirus as a carrier cellmay provide a novel efficient therapeutic approach for targeting the TC-1tumor model cell.

کلیدواژه ها:

Reovirus T3D ، Mesenchymal stem cells (MSCs) ، Carrier cell ، TC-1 cell ، Apoptosis

نویسندگان

Razieh Sadat Banijamali

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Hoorieh Soleimanjahi

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Sara Soudi

Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Hesam Karimi

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran