EFFECTS OF SYNBIOTIC SUPPLEMENTATION WITH EDUCATING LOW-CALORIE DIET ON GUT MICROBIOTA IN PATIENTS WITH METABOLIC SYNDROME

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 316

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

INC15_029

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Metabolic syndrome (MetS) is increasing in an alarming rate and great sum of non-communicable diseases like type2 diabetes mellitus (T2DM), cardiovascular diseases (CVDs) and some types of cancers originated from this syndrome. Thereafter, finding effective methods to control it considered of an importance in medical researches. We conducted this study to determine the effects of synbiotic supplementation with following low-calorie diet on reestablishment of balance in healthy probiotics (symbiosis) of gut microflora in patients with MetS.Methods: 108 subjects aged 25-65 year with MetS participated in this double-blind randomized clinical controlled trial. Subjects in Synbiotic group (n=52) were received 1000mg/day synbiotic supplement (Familact®, Zist Takhmir) and those in Placebo group were consumed the same amount of standard placebo capsules for 12 weeks. All the participant were educated how to follow a healthy, low-calorie diet. Real Time-polymerase chain reaction (Real Time-PCR) and PCR were used to determine the presence and count of two probiotics consisting of Lactobacillus and Bifidobacterium species in stool samples of subjects before and after the trial.Results: As regards amplification plots and melt curves obtained from Real Time-PCR and photos of Gel-Doc instrument, both groups illustrated a mild increment in population of Lactobacillus and Bifidobacterium in comparison with the beginning of the study.Conclusion: In overall, combination of synbiotic supplementation and following a healthy, low-calorie diet can end in modifying imbalance of gut microbiota (dysbiosis) through increasing gram-positive to gram-negative bacteria ratio in the small and particularly large intestine.

نویسندگان

Fateme Rahimi

Nutritional Sciences Department, Faculty of Nutrition and Food Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Yahya Pasdara

Nutritional Sciences Department, Faculty of Nutrition and Food Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Golbon Sohrab

Department of Clinical Nutrition and Dietetics, Shahid Beheshti University of Medical Sciences, Tehran,Iran.

Soheil Abbasi

Nutritional Sciences Department, Faculty of Nutrition and Food Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.