Prevalence of plasmid-mediated quinolone resistance and class 1 integron genes in multidrug-resistant community isolates of Klebsiellaspp.in Yazd

Background: Klebsiellaspp. Isolates are important opportunistic pathogens and the cause of nosocomial and community acquired infections. Plasmid-mediated quinolone resistance (PMQR) genes often carried by integrons, have a considerable contribution to multidrug-resistance (MDR) in these pathogens. We studied the prevalence of PMQR and integron genes in community isolates of Klebsiellaspp. In Yazd Central Laboratory. Methods: Two hundred and fifty outpatient’s isolates of Klebsiella were collected from Yazd Central Laboratory August 2015 and October 2017. Antibiotic susceptibility was determined against 17 antibiotics by disc diffusion according to CLSI protocols and minimum inhibitory concentrations (MIC) were determined for ciprofloxacin. Detection of qnrA, qnrB, qnrS, aac (6’)-Ib -cr, oqxAB and qepA genes was carried out by PCR. Results: Seventeen isolates (6.8%) were MDR, two of which were Klebsiella oxytoca and 15 were Klebsiella pneumoniae. MIC measurements revealed 11 ciprofloxacin-resistant (including two K. oxytoca isolates), three intermediately-resistant and three ciprofloxacin-susceptible isolates. The most prevalent PMQR gene was oqxAB (93.75%) followed by aac (6’)-ib-cr (50.0%), qnrB (25.0%) and qnrS (18.75%) butqnrA and qepA were not detected. Nine isolates carried multiple PMQR genes. Class1 integron was observed in 14 isolates (82.3%) including nine ciprofloxacin-resistant, two intermediately-resistant, and three susceptible isolates. Conclusions: Presence of integron-mediated MDR and multiple PMQR genes in community isolates of Klebsiellacan is an important reservoir for transmission of antibiotic resistance determinants among community strains

کلیدواژه ها:

Plasmid-mediated quinolone resistance ، PMQR ، Klebsiellaspp ، Integron ، Community isolates ، MDR