Prevalence and genotype determination of human papilloma virus infection among woman in hormozgan Province in south of Iran

Background and Aim: Human papillomavirus (HPV) is a well-known cause of cervical cancer, the second most frequent cancer in female population. Thus the aim of this study was to determine the prevalence of various genital human papilloma viruses (HPV) among women being subjected to routine pap smear test in Hormozgan province of Iran. Methods: One-Hundred Pap smears sample were collected through a descriptive cross-sectional method for six months from different parts of Hormozgan province. HPV DNA was then extracted. All samples were identified by nested PCR assay. The genotypes of positive specimens were determined by reverse hybridization using the INNO-LiPA HPV genotyping system. Results: Relying on the collected data, 14(14%) samples were detected positive for HPV DNA and 86(83.5 %) samples out of 100 samples were detected negative for HPV DNA. Totally, eight HPV genotypes were found: two types (16 and 53) were carcinogenic, or high risk genotypes and three genotypes (52, 53 and 66) were probably carcinogenic and three genotypes (6, 46,54, 61) were low risk. HPV-16 is the most prevalent type among high risk HPV in Hormozgan Province. Conclusion: So as high-risk HPV types has correlation with cervical cancer, there is daily increase in need of screening test for woman at risk of HPV infection. Also, based on our and other studies results, surely, approximation of HPV infection would give valuable data particularly to recommend the strategies for the screening of cervical cancer and HPV vaccination in Iran.

کلیدواژه ها:

Human papilloma virus ، Pap Smear ، Cervical cancer ، Nested PCR