Synthesis andCharactrization of Hg/Chitosan Nanocomposites:Antibacterial Effect Study

Abstract: The rapid and uncontrolled growth of microorganisms can lead to serious problems. With the development of nanotechnology over the last decade, golden opportunities have been created to discover the antibacterial effects of metallic nanoparticles. Metallic nanoparticles have an antibacterial effect, in addition to the inhibition effect of the particle, due to its small size, large surface area and large outer surface area. Scientists believe that nanoparticles can be used as an appropriate alternative used biochemicals.In the past two decades, the field of nanotechnology has grownexponentially since its birth and has made an immense impact onphysical, chemical, earth and biological sciences[1-3]. There hasbeen an immense extension of nanomaterial applications and usesas a result of basic and applied research from scientists all over theworld. One such class of nanomaterialsare metal oxide (MeO) and metalsulfides (MeS) nanoparticles (NPs), ranging in size from 1 to 100 nm and available in different shapes and sizes.Methods and Results:In this research, Hg/Chitosan nanocomposites synthesized by nanoemulsion assistance with electrospinning method. 50 mg of HgI2, 100mg polylactic acid and 100 mg chitosan were precursors to get started.To prepare 100 cc of the medium, weigh the hinton broth 2.1 grams, and in another 500 ml, it dissolves and dissolves. Then we make microbial leachate from the tested microorganisms and compare with the half McFarland their turbidity, to the extent that they are half McFarland. Then, with a micropipette of 2.5 microns from the lagoon, remove the bacteria on the plates, and finally place the plates in the incubator for 24 hours, and the next day the results are read.Antibacterial activity of Ag2S/chitosan nanocomposites was performed on 12 bacterial isolates include Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa, Serratia marcescens , Salmonella typhi, Acinetobacter baumannii, Citrobacter freundii, Proteus mirabilis, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis.The bacteria were propagated on nutreint Agar (Merck, Germany) at 37°C, and maintained at 4°C before useConclusions:In this work, research into the extent of preventing microbial growth is investigated after the construction of nanoparticles in the size and dispersion of the microbes. After making nanoparticles and investigating nanostructures on bacterial strains, we found that 32% of nanoparticles prevents the growth of bacteria in the control.

کلیدواژه ها:

Nanostructures ، Hg/ChitosanNanocomposites ، Antibacterial Properties ، Gram Positive and Negative Bacteria