The importance of cell culture as a new approach in personalized drug therapy of oral cancer

Background: Oral cancer is considered to be one of the most prevelant in neck and head cancers. Oral Squamous Cell Carcinoma (OSCC) consist more than 90% of oral cancers diagnosed. Up to now, evaluation of tumors is done by investigating formalin-fixed, paraffin-embedded and freezed tissue samples, which is basically a snapshot of cells and is not suitable for comprehensive studies. One of the approperiate approaches in treating cancers is use of cell culture in diagnosis and targeted therapy.In this study, the ten cell lines were produced from oral cancer tissues for studies on drug effectivenessMaterials and Methods: After obtaining tissue samples from oral cancer patients, cells were isolated using enzymatic digestion and explant culture. After quality control of cells, characterization and authentication of OSCC cells were determined by STR, karyotyping, species identification, growth, morphology and expression of CD326 and CD133 markers as squamous cell cancer markers.Results: Cells obtained in this study were epithelial-like and had population doubling time (PDT) 30±2.5 hours. Flowcytometry analysis showed that cells were positive for CD326 and CD133 markers. Also, all cells were negative for mycoplasma, bacteria and fungi contaminations. All cells were banked at Human and Animal Cell bank, Iranian Biological Resource Center.Conclusion: It can be gathered from the results of this study that the established cell lines would provide an extremely valuable in vitro models for studying carcinogenesis pathways of OSCC in Iranian population and it can be considered as a proper method in personalized drug therapy.

کلیدواژه ها:

oral cancer ، cell culture ، characterization ، authentication