Gold/polypyrrole/Graphene oxide nanocomposite modified electrode forsimultaneous determination of epinephrine, acetaminophen, and folic acid

Recently, electrochemical techniques using modified electrodes have been developed forsimultaneous determination of biomolecules. Simultaneous determination of epinephrine (EP),acetaminophene (AC), and folic acid (FA) is of greater value because of their coexistence inbiological systems [1]. Graphene has become one of the most exciting topics of research in the lastdecades, since it possesses several excellent attributes, including electronic, optical, and thermalproperties, chemical and mechanical stability, and large surface area [2]. Polypyrrole (PPy), is oneof the most promising Conducting polymers because of its ease of synthesis, good redox property,high conductivity, and excellent environmental stability [3].Gold nanoparticles, which are usuallyapplied to bimolecular immobilization, have many advantages such as excellent catalytic activity,huge surface area, effective mass transport, and hospitable environment [4].A novel biosensor graphene oxide (RGO)/ polypyrrole (PPy)/gold (Au) nanoparticlesnanocomposite modified glassy carbon electrode (GCE) was prepared for simultaneousdetermination of epinephrine (EP), acetaminophen (AC) and folic acid (FA). Cyclic voltammetryand differential pulse voltammetry methods were used to investigate the modified electrode for theelectrocatalytic oxidation of EP, AC, and FA in aqueous solutions. The separation of the oxidationpeak potentials for EP-AC and AC-FA was about 210 and 270 mV, respectively. The calibrationcurves obtained for EP, AC, and FA were in the ranges of 0.4-260, 0.6-210, and 0.7-160 μM,respectively. The detection limits (S/N=3) were 0.071, 0.070, and 0.069 μM for EP, AC, and FA,respectively. Field emission-scanning electron microscopy (FE-SEM), Fourier transform infraredspectroscopy (FT-IR), Raman spectroscopy (Raman), X-ray photoelectron spectroscopy (XPS) andX-ray diffraction (XRD) were employed to characterize the modified electrode.

کلیدواژه ها:

Epinephrine ، acetaminophen ، folic acid ، nanoparticles ، Simultaneous determination