Cloning and expression of l-Asparaginase gene from Aspergillus terreus in E.coli
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 356
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شناسه ملی سند علمی:
CFAS01_430
تاریخ نمایه سازی: 20 آبان 1397
چکیده مقاله:
In the present study, L-Asparaginase was isolated from fungal source (Aspergillus terreus) and it is used as the treatment of malignancies. The synthesis of L-asparaginase from two mutant strains, WF and 933 of Serratia marcescens is reported and different media composition alters the levels of enzyme production. The phenotype was conducted by using a light microscope. While the genotype of the strain was also identified. The 18S rDNA sequences of the strain were determined by PCR. The enzyme was purified using ammonium sulphate precipitation, Sephadex G100, CM-cellulose and DEAE Sephadex chromatography.. The molecular weight of the l-Asparaginase from E.coli was determined by SDS-PAGE gel electrophoresis and it was found to be a single protein band with approximately 43 kDa.
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نویسندگان
IMAN MOHAMMED JEBUR
Research Scholar, Department of pathological Analysis Techniques, AL-Kafeel university,Annajaf Ashraf, Iraq.
HAYDER HASAN RGB AL
Research Scholar, Department of pathological Analysis Techniques, AL-Kafeel university,Annajaf Ashraf, Iraq.
KARRAR NAJIM NAJAF
Research Scholar, Department of pathological Analysis Techniques, AL-Kafeel university,Annajaf Ashraf, Iraq.