Objective: Alteration in semen parameters is one of the indicators of infertility and sperm function disturbances. Changes in some trace elements are suggested as the causative factors for these alterations. ROS are also considered as an infertility agent which their abnormal amounts are supposed to be related with the trace elementslevels. The aim of this study is evaluation of Iron, Copper, Magnesium, zinc, and Calcium levels in seminal plasma of infertile men and their relations to ROS levels. Materials and Methods: Considering sperm parameters, 100 human semen samples of infertile men were selected in 4 equal groups including: 25 normozoos permia (control), 25 oligozoospermia, 25 asthenozoospermia, and 25 teratozoospermia.
Semen samples collection and analysis were performed according to WHO 2010 manual. Assessments of trace elements were accomplished using commercial biochemical kits, and the ROS levels were evaluated by Chemiluminescence technique (WHO 2010). Data analysis was performed using SPSS 16.0 for Windows, and P<0.05 was considered statistically significant. Results: Comparison of trace elements values and ROS levels between all groupsshowed no significant difference among them. Meanwhile, there were significant relations between some elements inside each group as: 1) in oligospermic group: Cawas positively correlated with all 4 other elements; Cu, Fe and Zn were all positively correlated with each other but their correlation with Mg was not statistically significant; 2) in teratospermic group: Ca was positively correlated with Fe and Mg, Cu with Zn, and Fe with Ca and Mg; 3) in asthenospermic group: Ca and Cu were positively correlated with all 4 other elements, Fe and Mg were positively correlated with all elements but Zn; 4) in normospermic group, Ca was positively correlated with Cu and Zn, and there was a positive correlated between Fe and Cu. In addition, the dispersion of Zn levels in teratospermic and oligospermic groups were too high in comparison to others.Conclusions: Despite of the great difference between the means of Zn levels in oligoand tereto-spermic groups against the other groups, there was no significant relation between the main sperm parameters (concentration, motility and morphology) and the levels of Fe, Zn, Cu, Mg, and Ca in seminal plasma, as well as ROS levels. It is arisen due to the large dispersion of Zn values in both oligo- and terato-spermic group. It is suggested to investigate the potential factors and mechanisms which could impact on the Zn level in oligospermia and teratospermia cases.