The Effect of combination therapy of Caffeine and Glibenclamide on Oxidative Stress in the heart Tissue among Streptozotocin-Induced Diabetic Rats

Background and Objective: The Oxidative stress caused by diabetes, shows an imbalance between free radicals and antioxidant defense system ;that causes severe apoptosis in heart cells. Glibenclamide is one of the materials wich is widely used in diabetes, but has a lot of side effects such as hypoglycemia and loss of consciousness; nowadays Caffeine used in various countries and has anti-oxidant effect;but the effect of caffeine on the therapeutic role of glibenclamide is undetermined. This study was conducted to survey the combination therapy effect of caffeine and glibenclamide on the catalase activity as an oxidative stress index in the heart tissue among Streptozotocin-Induced Diabetic Rats. Materials and Methods: In this experimental investigation, 32 male rats, weighing 200 - 250 g, were divided into four groups: 1: diabetic without treatment, 2: diabetic receiving treatment with glibenclamide, 3: diabetic receiving treatment with caffeine, and 4: diabetic receiving treatment with glibenclamide and caffeine. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). On the third day after the injection of streptozotocin to ensure the mice are diabetic, then by using glucometer we measured the blood sugar and A value of blood sugar above 250 mg/dl was chosen to be the references for deciding whether they had diabetes or not;diabetic rats chose for the next step ; Treated group with glibenclamide get daily 0.3cc, treated group with caffeine get daily 0.5cc and the group treated with glibenclamide and caffeine get daily respectively, 0.15cc and 0.25ccby intraperitoneal injection. In the second week after drug administration, rats were anesthetized and by opening the pericardial cavity removed the heart tissue and after homogenizing and centrifuging, by using supernatant,the Catalase activity of the heart was measured by Beer& sizer method. Quantitatives data were analyzed using one-way ANOVA. Results: First we knew that all the rats were diabetic. Analysis results showed a significant increase in the level of the catalase activity in the heart of the group wich was treated with glibenclamide than diabetic group (p<0/01); the use of caffeine alone in diabetic rats doesn’t make a significant increase in the catalase activity of the heart. The same condition was seen about using caffeine and glibenclamide simultaneously. Conclusion: It was found that using glibenclamide has beneficial effect on the level of catalase activity in heart, but caffeine or the combination doesn’t have a beneficial and sensible effect on the level of catalase activity in diabetic rat’s heart.

کلیدواژه ها:

Diabetes Mellitus ، Oxidative Stress ، heart ، Rat ، Caffeine ، Glibenclamide